Tag: 336.3612

Non-invasive drug delivery systems mediated by nanocarriers and molecular dynamics simulation for posterior eye disease therapeutics: Virtual screening, construction and comparison was written by Zhang, Tingting;Jiao, Xinyi;Peng, Xingru;Wang, Haitao;Zou, Yadan;Xiao, Yanyu;Liu, Rui;Li, Zheng. And the article was included in Journal of Molecular Liquids in 2022.Related Products of 2086-83-1 This article mentions the following:

Local deliver medication in a non-invasive pattern and attaining a long-term sustained release at ocular regions to minimize side effects is fascinating for treatment and precision medicine, but its rational design remains a challenge. The aim of this study was to analyze the effect of different delivery strategies on drug bioavailability in the posterior segment of the eye. In this study, the results of mol. dynamics (MD) revealed that quercetin (QUE) and curcumin (CUR) have shown superior affinity and tighter binding capacity for the active site of vascular endothelial growth factor (VEGF). Then, developing and comparing lipid-based nanoemulsion (NE) and polymer-based nanomicelles (NM) were modified with novel mPEG-CS to achieve adequate targeting and retention. The results of in vitro biol. properties showed that nano-preparations could be successfully absorbed by cells. The result of in vivo pharmacokinetics revealed that two kinds of nano-preparations could prominently enhance the ocular bioavailability of QUE and CUR to different degrees and have a certain sustained-release effect. More importantly, the results showed that the particle size of NE was smaller than that of NM, which would make the drug retention time in the eye short and result in drug loss. Compared with NE, the release rate of NM was slower, and the effect of improving the drug bioavailability was more significant. In addition, the hydrophobic inner core-hydrophilic shell structure of NM and the carbonyl group in Soluplus material played a protective role in maintaining the stability of the drug. In summary, NM has a more significant effect on improving the ocular absorption of drugs, and is expected to become a candidate nanocarrier for non-invasive drug delivery systems and used for the treatment of post-ocular diseases. In the experiment, the researchers used many compounds, for example, 9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium (cas: 2086-83-1Related Products of 2086-83-1).

9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium (cas: 2086-83-1) belongs to isoquinoline derivatives. Isoquinoline is a structural isomer of quinoline, which have antiseptic, antipyretic and anti-cyclic properties, and can be used as antimalarial drugs and for the preparation of other antimalarial medicine. Most traditional methods of generating isoquinoline do so via oxidative aromatization of dihydro- or tetrahydro-isoquinolines, usually offering some environmental or economic advantage.Related Products of 2086-83-1

Referemce:
Isoquinoline – Wikipedia,
Isoquinoline | C9H7N – PubChem

In Vitro Synergistic Inhibitory Activity of Natural Alkaloid Berberine Combined with Azithromycin against Alginate Production by Pseudomonas aeruginosa PAO1. was written by Zhao, Zhijing;Guo, Mengyu;Xu, Xiaona;Hu, Yue;Liu, Dongmei;Wang, Chunxia;Liu, Xinwei;Li, Yongwei. And the article was included in Oxidative medicine and cellular longevity in 2022.Recommanded Product: 9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium This article mentions the following:

Background: Berberine (BER) is a natural isoquinoline alkaloid which extensively been applied to treat bacterial infection in TCM for a long time. Alginate is an important component of Pseudomonas aeruginosa biofilm. Herein, we investigated the effects of berberine and azithromycin (AZM) on alginate in the biofilm of P. aeruginosa PAO1. Methods: The MIC and synergistic activity of BER and AZM against PAO1 were determined using the micro broth dilution and checkerboard titration methods, respectively. The effect of BER on PAO1 growth was evaluated using a time-kill assay. Moreover, the effects of BER, AZM, and a combination of both on PAO1 biofilm formation, kinesis, and virulence factor expression were evaluated at subinhibitory concentrations. The alginate content in the biofilm was detected using ELISA, and the relative expression of alginate formation-related genes algD, algR, and algG was detected by qRT-PCR. Results: Simultaneous administration of berberine significantly reduced the MIC of azithromycin, and berberine at a certain concentration inhibited PAO1 growth. Moreover, combined berberine and azithromycin had synergistic effects against PAO1, significantly reducing biofilm formation, swarming, and twitching motility, and the production of virulence factors. The relative expression of alginate-related regulatory genes algG, algD, and algR of the combined treatment group was significantly lower than that of the control group. Conclusion: In summary, berberine and azithromycin in combination had a significant synergistic effect on the inhibition of alginate production by P. aeruginosa. Further molecular studies are in great need to reveal the mechanisms underlying the synergistic activity between berberine and azithromycin. In the experiment, the researchers used many compounds, for example, 9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium (cas: 2086-83-1Recommanded Product: 9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium).

9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium (cas: 2086-83-1) belongs to isoquinoline derivatives. Quinoline is a catabolite of tryptophan, the basic structure of some antihypertensive drugs, such as the peripheral vasodilators prazosin and doxazosin. Other industrial applications include their use as corrosion inhibitors, preservatives, and as solvents for resins and terpenes.Recommanded Product: 9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium

Referemce:
Isoquinoline – Wikipedia,
Isoquinoline | C9H7N – PubChem

Inhibition of inflammasome activation via sphingolipid pathway in acute lung injury by Huanglian Jiedu decoction: An integrative pharmacology approach was written by Chen, Ying;Peng, Mingming;Li, Wei;Zhao, Mantong;Cao, Xia;Li, Chuanqiu;Zhang, Han;Yang, Mengru;Liang, Lanyuan;Yue, Yiming;Xia, Tianyi;Zhong, Renxing;Wang, Yi;Shu, Zunpeng. And the article was included in Phytomedicine in 2022.Name: 9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium This article mentions the following:

Acute lung injury (ALI) is a serious health issue which causes significant morbidity and mortality. Inflammation is an important factor in the pathogenesis of ALI. Even though ALI has been successfully managed using a traditiomal Chinese medicine (TCM), Huanglian Jiedu Decoction (HLD), its mechanism of action remains unknown. This study explored the therapeutic potential of HLD in lipopolysaccharide (LPS)-induced ALI rats by utilizing integrative pharmacol. Here, the therapeutic efficacy of HLD was evaluated using lung wet/dry weight ratio (W/D), myeloperoxide (MPO) activity, and levels of tumor necrosis factor (TNF-α), interleukin (IL)-1β and IL-6. Network pharmacol. predictd the active components of HLD in ALI. Lung tissues were subjected to perform Hematoxylin-eosin (H&E) staining, metabolomics, and transcriptomics. The acid ceramidase (ASAH1) inhibitor, carmofur, was employedto suppress the sphingolipid signaling pathway. HLD reduced pulmonary edema and vascular permeability, and suppressed the levels of TNF-α, IL-6, and IL-1β in lung tissue, Bronchoalveolar lavage fluid (BALF), and serum. Network pharmacol. combined with transcriptomics and metabolomics showed that sphingolipid signaling was the main regulatory pathway for HLD to ameliorate ALI, as confirmed by immunohistochem. anal. Then, we reverse verified that the sphingolipid signaling pathway was the main pathway involed in ALI. Finally, berberine, baicalein, obacunone, and geniposide were docked with acid ceramidase to further explore the mechanisms of interaction between the compound and protein. HLD does have a better therapeutic effect on ALI, and its mol. mechanism is better elucidated from the whole, which is to balance lipid metabolism, energy metabolism and amino acid metabolism, and inhibit NLRP3 inflammasome activation by regulating the sphingolipid pathway. Therefore, HLD and its active components can be used to develop new therapies for ALI and provide a new model for exploring complex TCM systems for treating ALI. In the experiment, the researchers used many compounds, for example, 9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium (cas: 2086-83-1Name: 9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium).

9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium (cas: 2086-83-1) belongs to isoquinoline derivatives. Quinoline and its derivatives are widely used as fungicides, biological agents, antibiotics, alkaloids, dyes, rubber chemicals and flavoring agents. The discovery of chloroquine, the most well-known drug containing this stent, has helped bring about decades of malaria control and treatment.Name: 9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium

Referemce:
Isoquinoline – Wikipedia,
Isoquinoline | C9H7N – PubChem

Multifaceted role of polyphenols in treatment and management of neurodegenerative diseases was written by Islam, Fahadul;Islam, Mohaimenul Md;Khan Meem, Atkia Farzana;Nafady, Mohamed H.;Islam, Rezaul Md;Akter, Aklima;Mitra, Saikat;Alhumaydhi, Fahad A.;Emran, Talha Bin;Khusro, Ameer;Simal-Gandara, Jesus;Eftekhari, Aziz;Karimi, Fatemeh;Baghayeri, Mehdi. And the article was included in Chemosphere in 2022.SDS of cas: 2086-83-1 This article mentions the following:

Neurodegenerative diseases (NDDs) are conditions that cause neuron structure and/or function to deteriorate over time. Genetic alterations may be responsible for several NDDs. However, a multitude of physiol. systems can trigger neurodegeneration. Several NDDs, such as Huntington, Parkinson, and Alzheimer are assigned to oxidative stress (OS). Low concentrations of reactive oxygen and nitrogen species are crucial for maintaining normal brain activities, as their increasing concentrations can promote neural apoptosis. OS-mediated neurodegeneration has been linked to several factors, including notable dysfunction of mitochondria, excitotoxicity, and Ca ²⁺stress. However, synthetic drugs are commonly utilized to treat most NDDs, and these treatments have been known to have side effects during treatment. According to providing empirical evidence, studies have discovered many occurring natural components in plants used to treat NDDs. Polyphenols are often safer and have lesser side effects. As, epigallocatechin-3-gallate, resveratrol, curcumin, quercetin, celastrol, berberine, genistein, and luteolin have p-values less than 0.05, so they are typically considered to be statistically significant. These polyphenols could be a choice of interest as therapeutics for NDDs. This review highlighted to discusses the putative effectiveness of polyphenols against the most prevalent NDDs. In the experiment, the researchers used many compounds, for example, 9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium (cas: 2086-83-1SDS of cas: 2086-83-1).

9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium (cas: 2086-83-1) belongs to isoquinoline derivatives. The isoquinoline structure occurs in a considerable number of alkaloids in widely separated plant families. They are also used to make oil-soluble dyes, food colorants, pharmaceuticals, pH indicators and other organic compounds. SDS of cas: 2086-83-1

Referemce:
Isoquinoline – Wikipedia,
Isoquinoline | C9H7N – PubChem

Screening of plant-based natural compounds as an inhibitor of FtsZ from Salmonella Typhi using the computational, biochemical and in vitro cell-based studies was written by Naz, Farah;Kumar, Mukesh;Koley, Tirthankar;Sharma, Priyanka;Haque, Muhammad Anzarul;Kapil, Arti;Kumar, Manoj;Kaur, Punit;Ethayathulla, Abdul Samath. And the article was included in International Journal of Biological Macromolecules in 2022.Reference of 2086-83-1 This article mentions the following:

Salmonella Typhi is emerging as a drug-resistant pathogen, particularly in developing countries. Hence, the progressive development of new antibiotics against novel drug targets is essential to prevent the spread of infections and mortality. The cell division protein FtsZ is an ideal drug target as the cell wall synthesis in bacteria is driven by the dynamic treadmilling nature of the FtsZ. The polymerization of the FtsZ provides the essential mech. constricting force and flexibility to modulate the cell wall synthesis. Any alteration in FtsZ polymerization leads to the bactericidal or bacteriostatic effect. In this study, we have evaluated the secondary metabolites of natural compounds berberine chloride, cinnamaldehyde, scopoletin, quercetin and eugenol as potential inhibitors of FtsZ from Salmonella Typhi (stFtsZ) using computational, biochem., and in vivo cell-based assays. Out of these five compounds, berberine chloride and cinnamaldehyde exhibited the best binding affinity of Kd = 7μM and 10μM, resp. and inhibit stFtsZ GTPase activity and polymerization by 70%. The compound berberine chloride showed the best MIC of 500μg/mL and 175μg/mL against gram-neg. and gram-pos. bacterial strains. The findings support that these natural compounds can be used as a backbone structure to develop a broad spectrum of antibacterial agents. In the experiment, the researchers used many compounds, for example, 9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium (cas: 2086-83-1Reference of 2086-83-1).

9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium (cas: 2086-83-1) belongs to isoquinoline derivatives. Quinoline is a catabolite of tryptophan, the basic structure of some antihypertensive drugs, such as the peripheral vasodilators prazosin and doxazosin. It protonates to form salts upon treatment with strong acids, such as HCl. It forms adducts with Lewis acids, such as BF3.Reference of 2086-83-1

Referemce:
Isoquinoline – Wikipedia,
Isoquinoline | C9H7N – PubChem

Constructing mesoporous Zr-doped SiO₂ onto efficient Z-scheme TiO₂/g-C₃N₄ heterojunction for antibiotic degradation via adsorption-photocatalysis and mechanism insight was written by Yu, Yutang;Hu, Xingyu;Li, Meng;Fang, Jianzhang;Leng, Chengmeng;Zhu, Ximiao;Xu, Weicheng;Qin, Jingjun;Yao, Lang;Liu, Zhang;Fang, Zhanqiang. And the article was included in Environmental Research in 2022.Computed Properties of C20H18NO4 This article mentions the following:

Novel modified-TiO₂/Zr-doped SiO₂/g-C₃N₄ ternary composite is fabricated via an in-situ grow of porous Zr-SiO₂ layer to TiO₂/g-C₃N₄ heterojunction, which exhibits well adsorption-photocatalytic performance under simulated solar light irradiation The nano-size mesoporous TiO₂ are dispersed on the lamellar g-C₃N₄, and the Zr-SiO₂ is in-situ fabricated onto the surface of g-C₃N₄ sheets. The adsorption occurs on the SiO₂ layers, and doping Zr element to SiO₂ enhances the adsorption of pollutants, while the photocatalytic reaction occurs on the valence band (VB) of TiO₂ and conduction band (CB) of g-C₃N₄, which gives reactive oxygen species of ·O^–2, h⁺, and ·OH for high efficient decomposition of antibiotics, i.e. berberine hydrochloride (98.11%), tetracycline (80.76%), and oxytetracycline (84.84%). The excellent adsorption capacity and Z-scheme photoinduced charge carrier migration behavior endowed the novel material with enhanced berberine hydrochloride (BH) removal in water, which approx. 2.5 and 3.8 folds than that of pure g-C₃N₄ and sole TiO₂, resp. Three degradation pathways are unraveled by LC-MS and theor. calculations Furthermore, the toxicity of intermediates was evaluated by the Toxicity Estimation Software Tool (T.E.S.T.), the result demonstrated a good application potential of M-TiO₂/Zr-SiO₂/g-C₃N₄ as an novel adsorptive photocatalyst. In the experiment, the researchers used many compounds, for example, 9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium (cas: 2086-83-1Computed Properties of C20H18NO4).

9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium (cas: 2086-83-1) belongs to isoquinoline derivatives. Quinoline is a catabolite of tryptophan, the basic structure of some antihypertensive drugs, such as the peripheral vasodilators prazosin and doxazosin. The Pomeranz–Fritsch reaction provides a method for the preparation of isoquinoline. This reaction uses a benzaldehyde and aminoacetoaldehyde diethyl acetal, which in an acid medium react to form isoquinoline.Computed Properties of C20H18NO4

Referemce:
Isoquinoline – Wikipedia,
Isoquinoline | C9H7N – PubChem

Effect of berberine on global modulation of lncRNAs and mRNAs expression profiles in patients with stable coronary heart disease was written by Han, Ye-Chen;Xie, Hong-Zhi;Lu, Bo;Xiang, Ruo-Lan;Li, Jing-Yi;Qian, Hao;Zhang, Shu-Yang. And the article was included in BMC Genomics in 2022.Computed Properties of C20H18NO4 This article mentions the following:

Berberine (BBR) is an isoquinoline alkaloid found in the Berberis species. It was found to have protected effects in cardiovascular diseases. Here, we investigated the effect the regulatory function of long noncoding RNAs (lncRNAs) during the treatment of stable coronary heart disease (CHD) using BBR. We performed microarray analyzes to identify differentially expressed (DE) lncRNAs and mRNAs between whole blood samples from 5 patients with stable CHD taking BBR and 5 no BBR volunteers. DE lncRNAs and mRNAs were validated by quant. real-time PCR. A total of 1703 DE lncRNAs and 912 DE mRNAs were identified. Kyoto Encyclopedia of Genes and Genomes pathway anal. indicated DE mRNAs might be associated with mammalian target of rapamycin and mitogen-activated protein kinase pathway. These pathways may be involved in the healing process after CHD. To study the relationship between mRNAs encoding transcription factors (DNA damage inducible transcript 3, sal-like protein 4 and estrogen receptor alpha gene) and CHD related de mRNAs, we performed protein and protein interaction anal. on their corresponding proteins. AKT and apoptosis pathway were significant enriched in protein and protein interaction network. BBR may affect downstream apoptosis pathways through DNA damage inducible transcript 3, sal-like protein 4 and estrogen receptor alpha gene. Growth arrest-specific transcript 5 might regulate CHD-related mRNAs through competing endogenous RNA mechanism and may be the downstream target gene regulated by BBR. Verified by the quant. real-time PCR, we identified 8 DE lncRNAs that may relate to CHD. We performed coding and non-coding co-expression and competing endogenous RNA mechanism anal. of these 8 DE lncRNAs and CHD-related DE mRNA, and predicted their subcellular localization and N⁶-methyladenosine modification sites. Our research found that BBR may affect mammalian target of rapamycin, mitogen-activated protein kinase, apoptosis pathway and growth arrest-specific transcript 5 in the process of CHD. Our research might provide novel insights for functional research of BBR. In the experiment, the researchers used many compounds, for example, 9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium (cas: 2086-83-1Computed Properties of C20H18NO4).

9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium (cas: 2086-83-1) belongs to isoquinoline derivatives. The isoquinoline structure occurs in a considerable number of alkaloids in widely separated plant families. Lewis acid activation and nucleophilic attack can produce multiple regioisomers in quinolines and pyridines; isoquinolines generally only undergo attack at the 1-position.Computed Properties of C20H18NO4

Referemce:
Isoquinoline – Wikipedia,
Isoquinoline | C9H7N – PubChem

Berberine-mediated REDD1 down-regulation ameliorates senescence of retinal pigment epithelium by interrupting the ROS-DDR positive feedback loop was written by Chen, Qingqiu;Xin, Guang;Li, Shiyi;Dong, Yuman;Yu, Xiuxian;Wan, Chengyu;Wei, Zeliang;Zhu, Yuda;Zhang, Kun;Wang, Yilan;Li, Fan;Zhang, Cuicui;Wen, E.;Li, Yulong;Niu, Hai;Huang, Wen. And the article was included in Phytomedicine in 2022.Category: isoquinoline This article mentions the following:

Accumulation of age-associated senescent cells accompanied with increased reactive oxygen species (ROS) and inflammatory factors contributes to the progression of age-related macular degeneration (AMD), the main cause of blindness in the elderly. Berberine (BBR) has shown efficacy in the treatment of age-related diseases including diabetes and obesity by decreasing ROS. However, the pharmacol. effect of BBR on alleviating retinal aging remains largely unknown. Our study aimed to investigate the pharmacol. effect of BBR as an anti-aging agent in retinal aging and its further mol. mechanisms. D-galactose (DG)-induced ARPE-19 cell senescence and retinal aging were employed to evaluate the anti-aging effect of BBR in vivo and in vitro. The siRNA transfection, Western-Blot analyses, SA-β-Gal assay and immunofluorescence were performed to investigate the potential mechanisms of BBR on anti-aging of RPE. In RPE-choroid of both natural aged and DG-induced accelerated aged mice, oxidative stress was increased along with the up-regulation of p21 expression, which was ameliorated by BBR treatment. BBR down-regulated the expression of REDD1 to decrease intracellular ROS content, attenuating DG-induced senescence in vitro and in vivo. Furthermore, p53 instead of HIF-1α was identified as the transcriptional regulator of REDD1 in DG-induced premature senescence. Importantly, NAC and BBR reversed the expression of p53 and the content of 8-OHdG, indicating that the pos. feedback loop of ROS-DNA damage response (DDR) was formed, and BBR interrupted this feedback loop to alleviate DG-induced premature senescence by reducing REDD1 expression. In addition, BBR restored DG-damaged autophagy flux by up-regulating TFEB-mediated lysosomal biosynthesis by inhibiting REDD1 expression, thereby attenuating cellular senescence. BBR down-regulates REDD1 expression to interrupt the ROS-DDR pos. feedback loop and restore autophagic flux, thereby reducing premature senescence of RPE. Our findings elucidate the promising effects of REDD1 on cellular senescence and the great potential of BBR as a therapeutic approach. In the experiment, the researchers used many compounds, for example, 9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium (cas: 2086-83-1Category: isoquinoline).

9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium (cas: 2086-83-1) belongs to isoquinoline derivatives. Being an analog of pyridine, isoquinoline is a weak base, with a pKa of 5.14. The Pomeranz–Fritsch reaction provides a method for the preparation of isoquinoline. This reaction uses a benzaldehyde and aminoacetoaldehyde diethyl acetal, which in an acid medium react to form isoquinoline.Category: isoquinoline

Referemce:
Isoquinoline – Wikipedia,
Isoquinoline | C9H7N – PubChem

Berberine-mediated REDD1 down-regulation ameliorates senescence of retinal pigment epithelium by interrupting the ROS-DDR positive feedback loop was written by Chen, Qingqiu;Xin, Guang;Li, Shiyi;Dong, Yuman;Yu, Xiuxian;Wan, Chengyu;Wei, Zeliang;Zhu, Yuda;Zhang, Kun;Wang, Yilan;Li, Fan;Zhang, Cuicui;Wen, E.;Li, Yulong;Niu, Hai;Huang, Wen. And the article was included in Phytomedicine in 2022.Category: isoquinoline This article mentions the following:

Accumulation of age-associated senescent cells accompanied with increased reactive oxygen species (ROS) and inflammatory factors contributes to the progression of age-related macular degeneration (AMD), the main cause of blindness in the elderly. Berberine (BBR) has shown efficacy in the treatment of age-related diseases including diabetes and obesity by decreasing ROS. However, the pharmacol. effect of BBR on alleviating retinal aging remains largely unknown. Our study aimed to investigate the pharmacol. effect of BBR as an anti-aging agent in retinal aging and its further mol. mechanisms. D-galactose (DG)-induced ARPE-19 cell senescence and retinal aging were employed to evaluate the anti-aging effect of BBR in vivo and in vitro. The siRNA transfection, Western-Blot analyses, SA-β-Gal assay and immunofluorescence were performed to investigate the potential mechanisms of BBR on anti-aging of RPE. In RPE-choroid of both natural aged and DG-induced accelerated aged mice, oxidative stress was increased along with the up-regulation of p21 expression, which was ameliorated by BBR treatment. BBR down-regulated the expression of REDD1 to decrease intracellular ROS content, attenuating DG-induced senescence in vitro and in vivo. Furthermore, p53 instead of HIF-1α was identified as the transcriptional regulator of REDD1 in DG-induced premature senescence. Importantly, NAC and BBR reversed the expression of p53 and the content of 8-OHdG, indicating that the pos. feedback loop of ROS-DNA damage response (DDR) was formed, and BBR interrupted this feedback loop to alleviate DG-induced premature senescence by reducing REDD1 expression. In addition, BBR restored DG-damaged autophagy flux by up-regulating TFEB-mediated lysosomal biosynthesis by inhibiting REDD1 expression, thereby attenuating cellular senescence. BBR down-regulates REDD1 expression to interrupt the ROS-DDR pos. feedback loop and restore autophagic flux, thereby reducing premature senescence of RPE. Our findings elucidate the promising effects of REDD1 on cellular senescence and the great potential of BBR as a therapeutic approach. In the experiment, the researchers used many compounds, for example, 9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium (cas: 2086-83-1Category: isoquinoline).

9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium (cas: 2086-83-1) belongs to isoquinoline derivatives. Being an analog of pyridine, isoquinoline is a weak base, with a pKa of 5.14. The Pomeranz–Fritsch reaction provides a method for the preparation of isoquinoline. This reaction uses a benzaldehyde and aminoacetoaldehyde diethyl acetal, which in an acid medium react to form isoquinoline.Category: isoquinoline

Referemce:
Isoquinoline – Wikipedia,
Isoquinoline | C9H7N – PubChem