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The reaction of an aromatic heterocycle with a proton is called a protonation. One of articles about this theory is 《Reactivity of the flavonic ring. I. Action of phenylhydrazine》. Authors are Venturella, Pietro; Bellino, Aurora; Cusmano, Sigismondo.The article about the compound:1,5-Diphenyl-1H-pyrazole-3-carboxylic acidcas:13599-22-9,SMILESS:OC(=O)C1=NN(C(=C1)C1=CC=CC=C1)C1=CC=CC=C1).Formula: C16H12N2O2. Through the article, more information about this compound (cas:13599-22-9) is conveyed.

A pyrazoline derivative, C21H18ON2 was obtained from flavanone (I) by action of NH2NHPh (II) and its structure was studied by an oxidizing demolition and a direct synthesis. Pure I (0.5 g.) in 5 cc. AcOH was treated with 0.3 g. II to yield after 12 hrs. at room temperature the phenylhydrazone (III), m. 146-7° (EtOH). I (0.5 g.) in 5 cc. AcOH and 0.3 g. II, refluxed 1 hr., the mixture cooled, filtered, and the product crystallized (EtOH) gave 3-(o-hydroxyphenyl)-1,5-diphenylpyrazoline (IV), m. 164-5°. I and II, melted in a test tube, gave IV. 2-Hydroxychalcone (V) (0.5 g.) in 10 cc. AcOH with 0.35 g. II after 2-3 days at room temperature gave IV. V (1 g.) in 15 cc. EtOH refluxed 3 hrs. with 0.6 g. II, cooled, and the product crystallized (EtOH) gave IV. V (0.2 g.) melted with II in excess gave IV. IV (0.5 g.) with 5 cc. Ac2O and 1 g. AcOMe refluxed 1.5 hrs., poured into H2O, and the product crystallized gave 3-(o-acetoxyphenyl)-1,5-diphenylpyrazoline (VI), m. 111-12°. 3-(o-Hydroxy-phenyl)-1,5-diphenylpyrazole (VII), m. 105-6°, was prepared from o-hydroxydibenzoylmethane and II. Analogously was prepared 5-(o-hydroxyphenyl)-1,3-diphenylpyrazole (VIII), m. 162-3°. VII (1 g.) in 20 cc. EtOH with metallic Na in excess refluxed 2 hrs. and the product crystallized gave IV. Boiling 1 g. IV in 30 cc. 0.5N NaOH with 7 g. KMnO4 in 50 cc. H2O gave 1,5-diphenyl-3-pyrazolecarboxylic acid. Ultraviolet and infrared spectra of IV, VI, VII and VIII were given.

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SDS of cas: 13599-22-9. So far, in addition to halogen atoms, other non-metallic atoms can become part of the aromatic heterocycle, and the target ring system is still aromatic. Compound: 1,5-Diphenyl-1H-pyrazole-3-carboxylic acid, is researched, Molecular C16H12N2O2, CAS is 13599-22-9, about Metronidazole containing pyrazole derivatives potently inhibit tyrosyl-tRNA synthetase: design, synthesis, and biological evaluation.

As an important enzyme in bacterial protein biosynthesis, tyrosyl-tRNA synthetase (TyrRS) has been an absorbing therapeutic target for exploring novel antibacterial agents. A series of metronidazole-based antibacterial agents has been synthesized and identified as TyrRS inhibitors with low cytotoxicity and significant antibacterial activity, especially against Gram-neg. organisms. Of the compounds obtained, I is the most potent agent which inhibited the growth of Pseudomonas aeruginosa ATCC 13525 (MIC = 0.98 μg/mL) and exhibited TryRS inhibitory activity (IC50 = 0.92 μM). Docking simulation was performed to further understand its potency. Membrane-mediated apoptosis in P. aeruginosa was verified by flow cytometry.

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The reaction of an aromatic heterocycle with a proton is called a protonation. One of articles about this theory is 《Preparation and properties of some bipyrazolyls》. Authors are Finar, I. L..The article about the compound:1,5-Diphenyl-1H-pyrazole-3-carboxylic acidcas:13599-22-9,SMILESS:OC(=O)C1=NN(C(=C1)C1=CC=CC=C1)C1=CC=CC=C1).Product Details of 13599-22-9. Through the article, more information about this compound (cas:13599-22-9) is conveyed.

The synthesis of 5,5′-dimethyl-1,1′-diphenyl-(I) and 1,1′,5,5′-tetraphenyl-3,3′-bipyrazolyl (II) was repeated, and the 3,5′-isomer (III) of the latter was isolated. Evidence was given for the orientations of these two isomers, and some 4,4′-disubstituted derivatives of I, II, and III were prepared (CO2Et)2 (IV) (36.5 g.) and 29 g. dry Me2CO were added during 25 min. to NaOMe (from 12.5 g. Na) under Et2O at 0°, the mixture was stirred for 2 days to give 21-38% octane-2,4,5,7-tetraone (V), yellow needles, m. 120-1° (from MeOH). IV (36.5 g.) and 1/2 of 16 g. PhCOMe were similarly treated, the other 1/2 added 4 hrs. later, and the mixture stirred 3 days to give 62-74% 1,6-diphenylhexane-1,3,4,6-tetraone (VI), yellow needles, m. 177-9°. V (3.4 g.) was heated with 4.3 g. PhNHNH2 in HOAc for 1 hr. to give 3.4 g. I, buff needles, m. 141-2°. VI (47.2 g.) was similarly treated 3-4 hrs. with 34.6 g. PhNHNH2 to give 35.2 g. II, m. 233°. The filtrate was diluted with H2O and then crystallized to yield 12.2 g. III, white needles, m. 135-6°. VI (58.8 g.) in HOAc was treated during 2.75 hrs. with 21.6 g. PhNHNH2 in HOAc, heated for a further 1.25 hrs., and set aside for 2 days to give after fractional crystallization unchanged starting material, II, and 3-(α-benzoylacetyl)-1,5-diphenylpyrazole (VII), yellow needles, m. 164-6.5°. VII on oxidation with alk. KMnO4 yielded 1,5-diphenylpyrazole-3-carboxylic acid (VIII), m. 185-6°. VII (2.9 g.) and 0.97 g. PhNHNH2 in HOAc heated 1 hr., and kept at room temperature overnight gave 2.4 g. II and 1 g. III. The infrared spectra of II and III were complex and similar. In general, the lowering of the symmetry as in III increases the number of bands. III in the 1600-650 cm.-1 region had 25 strong bands. Bischler’s method [Ber. 25, 3143(1892)] of preparing VIII was modified as follows: MeCOCH2CO2Et (30 g.) was refluxed 6 hrs. with 5.4 g. Na wire under Et2O, then 46 g. BzCH2Br in Et2O was added to maintain gentle reflux, then refluxed 2 hrs., and set aside overnight to give 56 g. AcCH(CH2Bz)CO2Et (IX) as a red oil. IX (12.4 g.) in EtOH was treated in the cold with 4.65 g. PhN2Cl, and then 16.4 g. NaOAc in H2O, the mixture set aside 24 hrs. in ice, and the oil which separated heated 15 min. with 6 g. NaOH in a little H2O to give 6.7 g. VIII. I in CHCl3 was treated with Br at room temperature to yield 4,4′-dibromo-5,5′-dimethyl-1,1′-diphenyl-3,3′-bipyrazolyl, rods, m. 159-60°. 4,4′-Dibromo-1,1′,5,5′-tetraphenyl-3,3′-bipyrazolyl, plates, m. 272-3°. 4,4′-Dibromo-1,1′,3′,5-tetraphenyl-3,5′-bipyrazolyl, white rosettes, m. 200-1°. II (13.2 g.) in HOAc and 26 cc. concentrated HCl was heated 2 hrs. with 2.4 g. paraformaldehyde to give the 4,4′-bis(chloromethyl) derivative, white needles, (6.2 g.), m. 274-6°. I (3.14 g.) in HOAc was heated 0.5 hrs. with 6.37 g. HgAc2 to yield 4.8 g. 4,4′-bis(acetoxymercuri) compound (X), white needles, m. 204-4.5° (aqueous HOAc). II (4.38 g.) similarly treated yielded after 5 hrs. refluxing 7.6 g. 4,4′-bis(acetoxymercuri)-1,1′,5,5′-tetraphenyl-3,3′-bipyrazolyl (XI), white powder, m. 271.5°. The mercuri compounds when treated with HOAc and Br at room temperature gave the corresponding 4,4′-di-Br compounds X refluxed with dilute HCl gave I; however, XI had to be refluxed for some time with HOAc containing concentrated HCl before II could be obtained.

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The three-dimensional configuration of the ester heterocycle is basically the same as that of the carbocycle. Compound: 1,5-Diphenyl-1H-pyrazole-3-carboxylic acid(SMILESS: OC(=O)C1=NN(C(=C1)C1=CC=CC=C1)C1=CC=CC=C1,cas:13599-22-9) is researched.Recommanded Product: 1-Bromo-2-pentyne. The article 《Esters of oxo acids of acetylene series. VI. Reaction of the esters of phenylethynylglyoxalic acid with hydrazine, hydrazide, and hydroxylamine》 in relation to this compound, is published in Zhurnal Organicheskoi Khimii. Let’s take a look at the latest research on this compound (cas:13599-22-9).

cf. CA 65, 10523b. Addition of N2H4.H2O to Et phenylethynylglyoxalate in EtOH gave 75% Et 3-phenylpyrazole-5-carboxylate, m. 139.5°; similarly were prepared: Pr ester, m. 110°; iso-Pr ester, m. 164°. Addition of RNHNH2 in Et2O to the appropriate ester of phenylethynylglyoxalic acid gave the following esters of 4-arylhydrazino-4-phenyl-3-buten-2-on-1-oic acids, RNHNHCPh:CCOCO2R’ (R and R’ shown, resp.): Ph, Et, 32%, m. 83°; Ph, iso-Pr (I) 65%, m. 124.5°; p-MeC6H4, iso-Pr, 32%, m. 77°; 0-MeC6H4, iso-Pr, 55%, m. 72.5°; Bz, iso-Pr (II) 87%, m. 125.5°; 0-O2NC6H4CO, iso-Pr, 78%, m. 137°; m-O2NC6H4CO, iso-Pr, 83%, m. 181°; p-O2NC6H4CO, iso-Pr, 88%, m. 175°. II heated in AcOH 2 hrs. gave 93% 1,5-diphenylpyrazole-3-carboxylic acid iso-Pr ester, m. 86.5-7°. I heated with aqueous alc. KOH 20 min. gave 1,5-diphenylpyrazole-3-carboxylic acid. Heating iso-Pr phenylethynylglyoxalate with HONH2.HCl in aqueous EtOH gave after addition of aqueous Na2CO3 over 9 hrs. 50% iso-Pr 3-phenylisoxazole-5-carboxylate, m. 65-6°. Similarly was prepared 29% Et 3-phenylisoxazole-5-carboxylate, m. 47°. Ir spectra were reported.

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In organic chemistry, atoms other than carbon and hydrogen are generally referred to as heteroatoms. The most common heteroatoms are nitrogen, oxygen and sulfur. Now I present to you an article called Transformation of 3-isoxazolecarboxylic acids into pyrazole derivatives. IV, published in 1940, which mentions a compound: 13599-22-9, mainly applied to , Synthetic Route of C16H12N2O2.

cf. C. A. 34, 7903.8. The transformation of 3-isoxazolecarboxylic acids into pyrazolonimines by fusion with PhNHNH2 may proceed by decarboxylation followed by ring closure of the resulting cyano ketone phenylhydrazone. To test this hypothesis the fusion was repeated in the presence of Natur Kupfer C (I) (or ordinary reduced Cu) so that, at the lower decarboxylation temperatures it might be possible to isolate the phenylhydrazone prior to ring closure and so shed some light on the mechanism of the reaction. A mixture of 1 g. of 5-phenyl-3-isoxazolecarboxylic acid (II), 1 g. I and 1 g. PhNHNH2 in 20 cc. alc. was boiled for a few min. over a free flame, filtered, alkalinized with Na2CO3, extracted free from PhNHNH2 with ether, acidified with dilute H2SO4, and extracted with ether. The residue from the evaporated extract gave 1,5-diphenyl-3-pyrazolecarboxylic acid (III), m. 185° (Et ester, m. 98°), decarboxylated by fusion to give 1,5-diphenylpyrazole, m. 55°, and identical with the known acid prepared by the action of PhNHNH2 on BzCH2COCO2H. A similar transformation of 5-methyl-3-isoxazolecarboxylic acid (IV) gave 1-phenyl-5-methyl-3-pyrazolecarboxylic acid, m. 136° (Me ester, m. 55°), decarboxylated to 1-phenyl-5-methylpyrazole, transformed into the known picrate, m. 98°. In these transformations alc. can be replaced by other solvents. In the absence of I or in the presence of PhNH2 instead of PhNHNH2 the isoxazolecarboxylic acid is recovered unchanged.

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The reaction of an aromatic heterocycle with a proton is called a protonation. One of articles about this theory is 《Enamines. V. Synthesis of 1-arylpyrazoles》. Authors are Fusco, Raffaello; Bianchetti, Giuseppe; Pocar, Donato.The article about the compound:1,5-Diphenyl-1H-pyrazole-3-carboxylic acidcas:13599-22-9,SMILESS:OC(=O)C1=NN(C(=C1)C1=CC=CC=C1)C1=CC=CC=C1).Computed Properties of C16H12N2O2. Through the article, more information about this compound (cas:13599-22-9) is conveyed.

cf. CA 56, 14018c. Enamines and halogenated hydrazones reacted to give pyrazole derivatives In anhyd, conditions 16.7 g. 1-morpholinocyclohex-l-ene (I), 10.1 g. NEt3, 150 mL. CHCl3, and 22.6 g. PhNHN: CClCO2Et added in 50 mL. CHCl3 was stirred and heated to 40° for 1 h. and kept overnight to give 65% 1-phenyl-3-carbethoxy-4,5,6,7-tetrahydroindazole (II) m. 103-4.5° (EtOH), hydrolyzed by alc. NaOH to 1-phenyl-4,5,6,7-tetrahydroindazole-3-carboxylic acid, m. 164° (AcOH). Similarly, the p-chlorophenyl compound, m. 139-40°, and its corresponding acid m. 218°. Treatment of I with PhNHN:CClAe gave 1-phenyl-3-acetyl-4,5,6,7-tetrahydroindazole, m. 95-6°; phenylhydrazone m. 216° (dilute AcOH). 1-Morpholino-4-methylcyclohex-1-ene gave the 5-Me deriv, of II, m. 129°, hydrolyzed to the corresponding acid m. 198°. 1-Phenyl-3-carbethoxy-4,5,6,7-tetrahydrocydohepta[c]pyrazole (III), m. 87% was prepared from 1-morpholinocyclohept-1-ene and was hydrolyzed to the corresponding acid, m. 166-8°. The 1-(p-chlorophenyl) compd, corresponding to III could not be isolated and was hydrolyzed to the acid, m. 206-7°. From 2 morpholino-3,4-dihydronaphthalene and PhNHN: CClCO2Et, 1-phenyl-3-carbethoxy-8,9-dihydronaphtho[1,2-c]pyrazole, m. 154-5°, was prepared, giving the acid, m. 170°, on hydrolysis: when decarboxylated at 240°, this gave 1-phenyl-8,9-dihydronaphtho[1,2-c]pyrazole, m. 118°. The compound formed from 1-morpholinocyclopent-1-ene and PhNHN:-CClCO2Et was an intermediate, C19H25N3O3, m. 130° (ale.), converted into the expected 1-phenvl-3-carbethoxy-4,5-dihydrocyclopenta[c] pyrazole, m. 143°. on boiling with AcOH followed by precipitation by addition of water. The corresponding acid, m. 211°; Me ester m. 139°. Similarly the 1-(p-chlorophenyl) compd, gave an adduet, C19H24ClN3O3, m. 114° cyclized to the pyrazole, m. 120-1°, which in turn was hydrolyzed to the corresponding free acid, m. 215-16°. Similarly were prepared 1-phenyl-3-carbethoxyindeno[1,2-c]-pyrazole, m. 162-3° (the acid m. 256°), 1-phenyl-3-carbethoxy-4-ethylpyrazole, m. 70° (free acid m. 138°), 1-phenyl-3-carbethoxy-4-n-amylpyrazole, m. 45-6° (free acid m. 121°), [the corresponding 1-(p-chlorophenyl) compd, m. 62° (free acid m. 115°)]. From 2-N-methyl-N-phenyl-amino-4-methylpent-l-ene, the ester prepd, was an oil, b2 186°, which gave 1-phenyl-5-isobutylpyrazole-3-carboxylic acid, m. 129°, on hydrolysis, α-(N-Methyl-N-phenyl)-aminostyrene and PhNHN:CClCO2Et after reaction, evapn, of solvent, steam distn, to remove PhAe, and hydrolysis gave 1,5-diphenylpyrazole-3-carboxylic acid, m. 183°. 1-Phenyl-3-carbethoxy-4-γ-methylaminoethyl-5-methylpyrazole oxalate, m. 174-5° (iso-PrOH), was prepared from 1,2-dimethyl-4,5-dihydropyrrole and gave the corresponding base, as a straw-colored oil, b1.5 220-40°, which on hydrolysis with 40% HBr gave the free carboxylic acid of the base after addition of Ag2O to precipitate AgBr, filtration, and passage of H2S to decomp, the Ag salt; the filtrate evaporated to dryness and the solid washed with MeOH and recrystallized from water gave the acid, m. 297°. 1-Phenyl-3-carbethoxy-4-γ-methylaminopropyl-5-methylpyrazole-HCl, m. 186-7° (iso-PrOH), 1-phenyl-4-γ-methylaminopropyl-5-methylpyrazolecarboxylic acid, m. 270-1° (5% aqueous EtCO-Me), and 1-phenyl-3-acetyl-4-γ-methylaminopropyl-5-methylpyrazole oxalate, m. 17,5-6°, were also prepared

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Application of 13599-22-9. Aromatic heterocyclic compounds can also be classified according to the number of heteroatoms contained in the heterocycle: single heteroatom, two heteroatoms, three heteroatoms and four heteroatoms. Compound: 1,5-Diphenyl-1H-pyrazole-3-carboxylic acid, is researched, Molecular C16H12N2O2, CAS is 13599-22-9, about Bcl-2/MDM2 Dual Inhibitors Based on Universal Pyramid-Like α-Helical Mimetics. Author is Wang, Ziqian; Song, Ting; Feng, Yingang; Guo, Zongwei; Fan, Yudan; Xu, Wenjie; Liu, Lu; Wang, Anhui; Zhang, Zhichao.

No α-helical mimetic that exhibits Bcl-2/MDM2 dual inhibition has been rationally designed due to the different helicities of the α-helixes at their binding interfaces. Herein, the authors extracted a one-turn α-helix-mimicking ortho-triarene unit from o-phenylene foldamers. Linking benzamide substrates with a rotatable C-N bond, the authors constructed a novel semirigid pyramid-like scaffold that could support its two-turn α-helix mimicry without aromatic stacking interactions and could adopt the different dihedral angles of the key residues of p53 and BH3-only peptides. On the basis of this universal scaffold, a series of substituent groups were installed to capture the key residues of both p53TAD and BimBH3 and balance the differences of the bulks between them. Identified by FP, ITC, and NMR spectroscopy, compound I that directly binds to Mcl-1, Bcl-2, and MDM2 with balanced submicromolar affinities was obtained. Cell-based experiments demonstrated its antitumor ability through Bcl-2/MDM2 dual inhibition simultaneously.

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The reaction of an aromatic heterocycle with a proton is called a protonation. One of articles about this theory is 《Luminescence phenomena in pyrazoline derivatives》. Authors are Straus, Fritz; Muffat, Carl; Heitz, W..The article about the compound:1,5-Diphenyl-1H-pyrazole-3-carboxylic acidcas:13599-22-9,SMILESS:OC(=O)C1=NN(C(=C1)C1=CC=CC=C1)C1=CC=CC=C1).SDS of cas: 13599-22-9. Through the article, more information about this compound (cas:13599-22-9) is conveyed.

cf. Auwers and Voss, C. A. 4, 586. The reaction whereby 1-phenylpyrazolines are obtained by the action of PhNHNH2 on certain aromatic ketones, owing to the extraordinary ease with which the hydrazones first formed rearrange, has been extended to a number of substituted ketones and hydrazines. PhCH:CHCOCH:CHPh reacts in this way, so that adopting Auwers’ scheme of classification, PhCH: CHCOR’, the grouping PhCH:CH falls in with aromatic and sec. or tert. aliphatic residues in favoring ring closure. The α- and β-naphthylhydrazones form the pyrazolines as easily as the Ph compounds NO2 groups in the p-position in the hydrazine residue and o- but not p-MeO groups in the ketone Ph residues make the ring closure more difficult. Halogens do not markedly hinder the pyrazoline formation as long as they are present only in the ketone or the hydrazine residue, but if present in both they render the hydrazones quite stable. These pyrazoline compounds show vivid fluorescence under the influence of Röntgen rays, almost as intense as that of BaPt(CN)4 in some cases, and not only in the solid state but in solution also, depending to a marked degree on the nature of the solvent; the alc. and AcOH solutions become only faintly luminescent while in CS2, C6H6 and CHCl3 the phenomenon is surprizingly intense. The compounds also show vivid fluorescence in diffuse daylight. The fluorescence produced in these 1,3,5-substituted pyrazolines by Röntgen rays differs from that produced by daylight, however, in that it is limited to those compounds in which an unsaturated residue (Ph or CO) is substituted in positions 3 and 5; where the limit for the daylight fluorescence lies has not been determined with certainty but as fluorescence of simple pyrazolines is nowhere mentioned, it is probably produced by a Ph group in position 1. The hydrazones first formed, which have been isolated in some cases, are intensely colored, even more so than their isomeric pyrazolines, but show no fluorescence. The pyrazoline ring, however, is not in itself the seat of the fluorescence phenomenon; the cyclic compounds resulting from the phenylhydrazones of cinnamylideneacetophenone and dicinnamylideneacetone, which do not contain a pyrazoline ring, also fluoresce under the influence of Röntgen rays. The intensity of the fluorescence depends on the nature of the substituents, increasing in the order below with the following substituents in positions 1, 3 and 5, resp.: Ph, Ph, Ph; Ph, p-Me2NC6H4CH:CH, p-Me2NC6H4; Ph, PhCH: CH, Ph; Ph, p-ClC6H4, p-ClC6H4; Ph, p-MeOC6H4CH:CH, p-MeOC6H4; Ph, p-ClC6H4CH:CH, p-ClC6H4; p-BrC6H4, p-ClC6H4CH:CH, p-ClC6H4. O2NC6H4 in position 1 destroys the fluorescence under the rays but not in daylight; 1-α- and β-C10H7 derivatives fluoresce. This fluorescence under Röntgen rays is not limited to these pyrazolines, however; it is found, partly in conjunction with ordinary fluorescence, in Ph-substituted ethylenes, butadienes and hexatrienes; the Ph-substituted acetylenes show it faintly; Ph3CH very brightly; CPh4 not at all; anthracene and retene very faintly; ms-dibromoanthracene intensely; hydrocollidinedicarboxylic ester quite strongly. The constitutions of the following hydrazones and pyrazolines were determined by the methods of A. and V. 1,5-Diphenyl-3-styrylpyrazoline, PhN.N:C(CH:CHPh).CH2.CHPh, m. 147-8° (Minunni, Gazz. chim. ital. 29, II, 398(1899)), evaporated to dryness with 2% KMnO4, gave BzOH and 1,5-diphenylpyrazole-3-carboxylic acid, needles from H2O, m. 183°; the pyrazoline is unchanged by Na-Hg and by boiling with AcOH; it dissolves in H2SO4 with golden yellow color and gives with FeCl3 a green color changing to blue. 1-α-Naphthyl-3-styryl-5-phenylpyrazoline, obtained by filtering a hot solution of 5 g. α-C10H7NHNH2.HCl and 2.7 g. KOAc in 200 g. absolute alc. from the precipitated KCl, boiling 1 hr. with 5 g. (PhCH:- CH)2CO in 100 cc. alc., then 0.5 hr. longer with charcoal, filtering, concentrating in vacuo to 0.5 its volume, pouring off the alc. after cooling and drying in vacuo over H2SO4, yellow needles with green fluorescence from absolute alc. containing some AcOEt, m. 164°. β-Isomer, yellow, felted needles with faint green fluorescence from AcOEt, m. 195°. 1-p-Bromophenyl-3-styryl-5-phenylpyrazoline, from 4 g. (PhCH:CH)2CO allowed to stand 2 days with 5 g. p-BrC6H4NHNH2 in 20 cc. AcOH, yellow needles with green fluorescence from AcOEt, m. 177°, soluble in H2SO4 with dirty green color changing to deep green on addition of FeCl3, unchanged by Na-Hg or boiling AcOH. From 4.5 g. (PhCH:CH)2CO boiled 3 hrs. with 3 g. p-O2NC6H4NHNH2 in alc. and a little AcOH and allowed to stand several days is obtained dibenzalacetone p-nitrophenylhydrazone, yellow-red leaflets from C6H6, m. 173°, soluble in H2SO4 with red-yellow color; 1.7 g. in 20 cc. AcOEt and 10 cc. AcOH reduced with 60 g. Na-Hg gave 0.5 g. crude solid p-C6H4(NH2)2, while boiling 1 hr. in 20 parts AcOH converts it into 1-p-nitrophenyl-3-styryl-5-phenylpyrazoline, yellow-red crystals with intense green fluorescence from AcOEt, m. 204-5°, soluble in H2SO4 with red-violet color hardly changed by FeCl3. o,o’-Dimethoxydibenzalacetone phenyl-hydrazone, brown-yellow warts from alc., m. 142°, reduced by Na-Hg in EtOH-AcOH to PhNH2 and rearranged by boiling AcOH into 1-phenyl-3-0-methoxystyryl-5-0-methoxyphenylpyrazoline, light yellow crystals from alc. (in which they form a solution with green-blue fluorescence), m. 153-4.5°, soluble in H2SO4 with red-yellow color changed to blue by FeCl3, gives no PhNH2 with Na-Hg. 1-Phenyl-3-p-methoxystyryl-5-p-methoxyphenylpyrazoline, from 5 g. dianisalacetone and 5 g. PhNHNH2 boiled 2 hrs. in 50 cc. C6H6, white-yellow leaflets (appearing almost green from their intense fluorescence) from AcOEt, m. 159°; it is more easily obtained by boiling the ketone and hydrazine 5 min. in 10 parts AcOH. 1-Phenyl-3-p-dimethylaminostyryl-5-p-dimethyl-aminophenylpyrazoline, yellow needles with extraordinarily intense green fluorescence from AcOEt, m. 192°. o,o’-Dichlorodibenzalacetone, obtained in rather poor yield by boiling 5 g. o-ClC6H4CHO and 1 g. Me2CO in 60 cc. alc. 15 min. with 3 cc. of 5% NaOMe, yellow needles from MeOH, m. 125°; p-bromophenylhydrazone, dark yellow, crystals from MeOH, m. 145°. 1-Phenyl-3-0-chlorostyryl-5-0-chlorophenylpyrazoline, yellow needles from alc. (the solution shows green fluorescence), m. 145°, soluble in H2SO4 with yellow-green color turned dark by FeCl3, unchanged by boiling AcOH. 1-Phenyl-3-p-chlorostyryl-5-p-chlorophenylpyrazoline, fine, yellow needles with strong green fluorescence from AcOEt, m. 212°, gives no PhNH2 with Na-Hg, gives so faint a reaction with FeCl3 in H2SO4 as not to reveal the presence of a pyrazoline. p,p-Dichlorodibenzalacetone p-bromophenylhydrazone, from 5 g. of the ketone and 3.5 g. of the hydrazine shaken 3 days, protected from the light, in 50 cc. AcOH yellow needles from C6H6, m. 183°, stable only in H, quickly turns brown in the air, soluble in H2SO4 with yellow color changed transiently green, then brown, by FeCl3, reduced by Na-Hg in EtOH-AcOH to p-BrC6H4NH2 and converted by boiling AcOH into 1-p-bromophenyl-3-p-chlorostyryl-5-p-chlorophenylpyrazoline, yellow needles with intense green fluorescence from alc., m. 173-4°, soluble in H2SO4 with yellow color changed to deep green by FeCl3, unchanged by Na-Hg. Methyl 1-phenyl-3-β-carbomethoxyvinylpyrazoline-5-carboxylate, PhN.N:C(CH:CHCO2Me).CH2.CHCO2Me, from CO(CH:CHCO2Me)2 and PhNHNH2 in boiling C6H6, yellow leaflets with green fluorescence from AcOEt, m. 153°, soluble in H2SO4 with red-yellow color changed to dark blue by FeCl3 (yield rather poor), unchanged by boiling AcOH. Ethyl ester, yellow leaflets with green fluorescence, m. 92.5°, hydrolyzed by aqueous alc. NaOH on the H2O bath to the free dicarboxylic acid, yellow needles, m. 204° (decomposition); Pb salt, yellow, finely granular precipitate Dimethyl ketopentadienedicarboxylate phenylmethylhydrazone, from the ester and the hydrazine in AcOH at 40°, dark red, refractive crystals from MeOH, m. 105°. Diethyl ester p-bromophenylhydrazone, deep red-yellow needles from MeOH, m. 134°. From 5 g. of A. and V.’s PhCH:CHCH: CHC(:NNHPh)Ph boiled 1 hr. with 20 cc. AcOH is obtained an isomer (a), probably PhN.N:CPh.CH:CH.CH2.CHPh or PhN.N:CPh.CH2.CH:CH.CHPh, warts with faint fluorescence from AcOH, m. 123-4°, soluble in H2SO4 with green-blue color changed to green by FeCl3, while the hydrazone dissolves with yellow color changed to brown by FeCl3; Na-Hg gives no PhNH2 but resinifies the substance. PhCH:CHCH: CHC(:NNHPh)Me dissolves in H2SO4 with red-yellow color changed to dirty green by FeCl3, yields PhNH2 with Na-Hg and is converted by boiling AcOH into an oil strongly fluorescing in C6H6 and whose red-yellow solution in H2SO4 is turned blue-red by FeCl3, while Na-Hg gives no PhNH2. (PhCH:CHCH:CH)2O and PhNHNH2 heated in AcOH and then in alc. give orange-yellow needles from alc. and AcOEt, m. 142° (Diel and Einhorn, Ber. 18, 2,325 (1885), describe a “”phenylhydrazone”” as m. 155°), dissolves in H2SO4 with orange-red color changing to blue on standing 10 sec. or adding FeCl3, gives no PhNH2 with Na-Hg but is resinified, is unchanged by AcOH. Evidently the substance is not a hydrazone but a cyclic compound analogous to (a) above. On oxidation with KMnO4 BzOH was the only product which could be isolated.

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Here is a brief introduction to this compound(13599-22-9)Reference of 1,5-Diphenyl-1H-pyrazole-3-carboxylic acid, if you want to know about other compounds related to this compound(13599-22-9), you can read my other articles.

Most of the compounds have physiologically active properties, and their biological properties are often attributed to the heteroatoms contained in their molecules, and most of these heteroatoms also appear in cyclic structures. A Journal, Article, ACS Medicinal Chemistry Letters called Design and Evaluation of Bispidine-Based SARS-CoV-2 Main Protease Inhibitors, Author is Shcherbakov, Dmitriy; Baev, Dmitriy; Kalinin, Mikhail; Dalinger, Alexander; Chirkova, Varvara; Belenkaya, Svetlana; Khvostov, Aleksei; Krut’ko, Dmitry; Medved’ko, Aleksei; Volosnikova, Ekaterina; Sharlaeva, Elena; Shanshin, Daniil; Tolstikova, Tatyana; Yarovaya, Olga; Maksyutov, Rinat; Salakhutdinov, Nariman; Vatsadze, Sergey, which mentions a compound: 13599-22-9, SMILESS is OC(=O)C1=NN(C(=C1)C1=CC=CC=C1)C1=CC=CC=C1, Molecular C16H12N2O2, Reference of 1,5-Diphenyl-1H-pyrazole-3-carboxylic acid.

For the first time, derivatives of 3,7-diazabicyclo[3.3.1]nonane (bispidine) were proposed as potential inhibitors of the SARS-CoV-2 main viral protease (3-chymotrypsin-like, 3CLpro). Based on the created pharmacophore model of the active site of the protease, a group of compounds were modeled and tested for activity against 3CLpro. The 3CLpro activity was measured using the fluorogenic substrate Dabcyl-VNSTLQSGLRK(FAM)MA; the efficiency of the proposed approach was confirmed by comparison with literature data for ebselen and disulfiram. The results of the experiments performed with bispidine compounds showed that 14 compounds exhibited activity in the concentration range 1-10μM, and 3 samples exhibited submicromolar activity. The structure-activity relationship studies showed that the mols. containing a carbonyl group in the ninth position of the bicycle exhibited the maximum activity. Based on the exptl. and theor. results obtained, further directions for the development of this topic were proposed.

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Reference:
Isoquinoline – Wikipedia,
Isoquinoline | C9H7N – PubChem