26947-41-1, 3-Isoquinolinecarbonitrile is a isoquinoline compound, ?involved in a variety of chemical synthesis. Rlated chemical reaction is continuously updated,26947-41-1
General procedure: Cells were streaked onto LBamp plate from frozen stocks of E. coli JM109 (PVL 1343 +MS13) containing NDO2 and incubated at 37¡ãC for 12h. Single colonies were selected for preculture preparation. A sterilized culture flask containing 10mL of LBamp+kan medium was inoculated with a single colony of E. coli JM109 (PVL 1343 +MS13) and the preculture was grown on an orbital shaker at 37¡ãC for 12hat 180rpm. 200mL of MSB medium containing 300mgL?1 thiamine, 4mLL?1 ampicillin, 2mLL?1 kanamycin (kan) and 20mL 20percent glucose, was transferred into 1L sterile shake flask and was inoculated with 2mL of preculture and resulting culture was grown at 30¡ãC until the culture turbidity reached 1.5 to 2.0at 660nm. Cells were induced with salicylic acid (as dioxane solution, 2mML?1 of MSB) and were grown for 2.5h under same conditions. Culture was centrifuged at 4500rpm (3736g) for 15minat 4¡ãC and supernatant was separated from cells. The supernatant was decanted and cells were resuspended in fresh MSB medium containing 300mgL?1 thiamine, 4mLL?1 ampicillin, 2mLL?1 kanamycin and 20mL 20percent glucose. An aliquot amount of substrate was added either directly or as a solution in 1,4-dioxane. Conversion of substrate was monitored with HPLC and the biotransformation was stopped when HPLC showed no further decrease in substrate peak or increase in product peak (18?24h). (0028) After completion of biotransformation, the biomass was removed by centrifugation. The supernatant was concentrated at 30¡ãC to 5?10mL under reduced pressure. The concentrate was stirred with acid-free EtOAc for 30?60min. Acid free EtOAc was prepared by stirring with saturated solution of Na2CO3 at low temperature. Organic layer was separated and the concentrate was again extracted with EtOAc. The combined organic layers were dried over Na2SO4 and the solvent was evaporated at reduced pressure at 40¡ãC. The crude material was analyzed by 1H NMR. Purification was performed using an automated MPLC with fraction collector.
The synthetic route of 26947-41-1 has been constantly updated, and we look forward to future research findings.
Reference£º
Article; Zia, Muhammad Farooq; Vasko, Agnes G.; Riedl, Zsuzsanna; Hametner, Christian; Hajos, Gyoergy; Mereiter, Kurt; Mihovilovic, Marko D.; Tetrahedron; vol. 72; 46; (2016); p. 7348 – 7355;,
Isoquinoline – Wikipedia
Isoquinoline | C9H7N – PubChem