Fluorescent cytochemistry of nucleus and chromosomes after fluorine chromization with berberine sulfate was written by Khadzhiolov, A. I.;Tsvetkova, E.;Vulkov, I.;Gitsov, L.;Cholakova, I.;Marinov, M.;Tsvetkov, I.. And the article was included in Archives de l’Union Medicale Balkanique et Bulletin de l’Union Medicale Balkanique in 1974.Quality Control of 9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium sulfate(2:1) This article mentions the following:
F chromization with berberine sulfate was performed on blood cells, cells from lymphatic nodes, and chromosome preparations Chromatin acquired a brilliant golden yellow color. There were regions in the nodes or even entire nodes almost completely without fluorescence; these were assumed to be undifferentiated or neoplastic cells containing a large amount of euchromatin. Similar differences in the fluorescence of deoxynucleoprotein complexes were observed in metaphasic and meiotic chromosomes. The regions with an intense golden yellow coloration probably corresponded to the localization of satellite or repetitive DNA. It was possible to distinguish quant. between active and nonactive DNA chromatin. In the experiment, the researchers used many compounds, for example, 9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium sulfate(2:1) (cas: 316-41-6Quality Control of 9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium sulfate(2:1)).
9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium sulfate(2:1) (cas: 316-41-6) belongs to isoquinoline derivatives. Quinoline is a catabolite of tryptophan, the basic structure of some antihypertensive drugs, such as the peripheral vasodilators prazosin and doxazosin. The Pomeranz鈥揊ritsch reaction provides a method for the preparation of isoquinoline. This reaction uses a benzaldehyde and aminoacetoaldehyde diethyl acetal, which in an acid medium react to form isoquinoline.Quality Control of 9,10-Dimethoxy-5,6-dihydro-[1,3]dioxolo[4,5-g]isoquinolino[3,2-a]isoquinolin-7-ium sulfate(2:1)
Referemce:
Isoquinoline – Wikipedia,
Isoquinoline | C9H7N – PubChem