Andreucci, Michele published the artcileReversal of radiocontrast medium toxicity in human renal proximal tubular cells by white grape juice extract, Formula: C11H9I3N2O4, the main research area is radiocontrast medium nephrotoxicity radiotoxicity Vitis juice extract radioprotectant; Cell injury; Kinase; Renal cell; Signaling; Vitis spp..
Radiocontrast media (RCM)-induced nephrotoxicity (CIN) is a major clin. problem accounting for 12% of all hospital-acquired cases of acute kidney injury. The pathophysiol. of CIN is not well understood, but direct toxic effects on renal cells have been postulated as contributing to CIN. We have investigated the effect of a white grape (Vitis vinifera) juice extract (WGJe) on human renal proximal tubular (HK-2) cells treated with the radiocontrast medium (RCM) sodium diatrizoate. WGJe caused an increase in phosphorylation of the prosurvival kinases Akt and ERK1/2 in HK-2 cells. Treatment of HK-2 cells with 75 mg I/mL sodium diatrizoate for 2.5 h and then further incubation (for 27.5 h) after removal of the RCM caused a drastic decrease in cell viability. However, pre-treatment with WGJe, prior to incubation with diatrizoate, dramatically improved cell viability. Anal. of key signaling mols. by Western blotting showed that diatrizoate caused a drastic decrease in phosphorylation of Akt (Ser473), FOXO1 (Thr24) and FOXO3a (Thr32) during the initial 2.5 h incubation period, and WGJe pre-treatment caused a reversal of these effects. Further anal. by Western blotting of samples from HK-2 cells cultured for longer periods of time (for up to 27.5 h after an initial 2.5 h exposure to diatrizoate with or without WGJe pre-treatment) showed that WGJe pre-treatment caused a neg. effect on phosphorylation of p38, NF-κB (Ser276) and pERK1/2 while having a pos. effect on the phosphorylation of Akt, FOXO1/FOXO3a and maintained levels of Pim-1 kinase. WGJe may alleviate RCM toxicity through modulation of signaling mols. that are known to be involved in cell death and cell survival and its possible beneficial effects should be further investigated.
Chemico-Biological Interactions published new progress about Cytotoxicity. 117-96-4 belongs to class isoquinoline, name is Diatrizoic Acid, and the molecular formula is C11H9I3N2O4, Formula: C11H9I3N2O4.
Referemce:
Isoquinoline – Wikipedia,
Isoquinoline | C9H7N – PubChem