Kishore, M. V. published the artcileDevelopment of RP-HPLC method for the determination of N-methylurea content in methohexital drug substance and its validation, Application of 1-Methylurea, the main research area is N methylurea methohexital reversed phase high performance liquid chromatog.
A simple, sensitive and rapid RP-HPLC method developed and validated for the determination of residual impurity N-Methylurea at low level in Methohexital drug substance. In this method column conditions are established and optimized on Atlantis dC18, 250 mm × 4.6 mm, 5μm column with oven temperature maintaining at 40°C. 0.02M Potassium Phosphate buffer was chosen as mobile phase A and acetonitrile was selected as mobile phase B in gradient reverse phase mode. Chromatog. parameters such as flow rate of mobile phase was maintained at 0.7 mL/min, 50μl of injection volume, total runtime 35 min, were established and detected the analyte at 205 nm in the present methodol. Based on validation data, the method is found to be specific, sensitive, accurate and precise. The Limit of detection (LOD) and Limit of quantification (LOQ) for this impurity is found to be 0.002%weight/weight and 0.005%weight/weight resp. The average recovery obtained was 96.9% at four levels in twelve determinations for N-Methylurea in Methohexital drug substance. This method can be used as good quality control tool for quantization of N-Methylurea at low level. The exptl. results are discussed in detail in this research paper.
International Journal of Pharmacy and Pharmaceutical Research published new progress about Anesthetics. 598-50-5 belongs to class isoquinoline, name is 1-Methylurea, and the molecular formula is C2H6N2O, Application of 1-Methylurea.
Referemce:
Isoquinoline – Wikipedia,
Isoquinoline | C9H7N – PubChem