Hughes, David T. published the artcileFunctional and nonfunctional forms of CquiOR91, an odorant selectivity subunit of Culex quinquefasciatus, Related Products of isoquinoline, the main research area is Culex odorant receptors 91 selectivity; Xenopus oocytes; electrophysiology; insect; olfaction; polymorphism; receptor.
In Culex quinquefasciatus, CquiOR91 is the ortholog of 2 larvae-specific odorant receptors (ORs) from Anopheles gambiae (AgamOr40, previously shown to respond to several odorant ligands including the broad-spectrum repellent N,N-diethyl-3-methylbenzamide, DEET) and Aedes aegypti (AaegOr40). When we cloned full-length CquiOR91 from a Culex quinquefasciatus larval head RNA sample, we found 2 alleles of this OR, differing at 9 residues. Functional anal. using the Xenopus oocyte expression system and 2-electrode voltage clamp electrophysiol. revealed one allele (CquiOR91.1) to be nonfunctional, whereas the other allele (CquiOR91.2) was functional. Receptors formed by CquiOR91.2 and CquiOrco responded to (-)-fenchone, (+)-fenchone, and DEET, similar to what has been reported for AgamOr40. We also identified 5 novel odorant ligands for the CquiOR91.2 + CquiOrco receptor: 2-isobutylthiazole, veratrole, eucalyptol, d-camphor, and safranal, with safranal being the most potent. To explore possible reasons for the lack of function for CquiOR91.1, we generated a series of mutant CquiOR91.2 subunits, in which the residue at each of the 9 polymorphic residue positions was changed from what occurs in CquiOR91.2 to what occurs in CquiOR91.1. Eight of the 9 mutant versions of CquiOR91.2 formed functional receptors, responding to (-)-fenchone. Only the CquiOR91.2 Y183C mutant was nonfunctional. The reverse mutation (C183Y) conferred function on CquiOR91.1, which became responsive to (-)-fenchone and safranal. These results indicate that the “”defect”” in CquiOR91.1 that prevents function is the cysteine at position 183.
Chemical Senses published new progress about Aedes aegypti. 21834-92-4 belongs to class isoquinoline, name is 5-Methyl-2-phenylhex-2-enal, and the molecular formula is C13H16O, Related Products of isoquinoline.
Referemce:
Isoquinoline – Wikipedia,
Isoquinoline | C9H7N – PubChem